![]() ![]() A typical illuminator lamp and housing is illustrated in Figure 1. Lamp voltage is controlled through a variable rheostat that is commonly integrated into the microscope stand. The most common source for today's microscopes is an incandescent tungsten-halogen bulb positioned in a reflective housing that projects light through the collector lens and into the substage condenser. Modern microscopes usually have an integral light source that can be controlled to a relatively high degree. Unfortunately, these methods did not provide reliable illumination and frequently the area of field illumination greatly exceeded the numerical aperture of the objective, causing glare and flooding. They often employed rather ingenious methods of gathering light, such as reflection from a large white board or scattering of sunlight on a cloudy day. Köhler Microscope Illumination Light Sources for Optical MicroscopyĮarly microscopists relied on oil lamps and natural sunlight to provide an external source of illumination for their primitive (but often remarkably accurate) microscopes. ![]() Molecular Expressions Microscopy Primer: Anatomy of the Microscope - Light Sources for Optical Microscopy ![]()
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